Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 72, Issue 2, Pages 157-165Publisher
ELSEVIER
DOI: 10.1016/j.mimet.2007.11.010
Keywords
biofilm quantification; microtiter plate; crystal violet; resazurine; FDA; XTT
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In the present study six assays for the quantification of biofilms formed in 96-well microtiter plates were optimised and evaluated: the crystal violet (CV) assay, the Syto9 assay, the fluorescein diacetate (FDA) assay, the resazurin assay, the XTT assay and the dimethyl methylene blue (DMMB) assay. Pseudomonas aeruginosa, Burkholderia cenocepacia, Staphylococcus aureus, Propionibacterium acnes and Candida albicans were used as test organisms. In general, these assays showed a broad applicability and a high repeatability for most isolates. In addition, the estimated numbers of CFUs present in the biofilms show limited variations between the different assays. Nevertheless, our data show that some assays are less suitable for the quantification of biofilms of particular isolates (e.g. the CV assay for P aeruginosa). (C) 2007 Elsevier B.V. All rights reserved.
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