Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 72, Issue 3, Pages 321-325Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2007.12.015
Keywords
digitonin; Coxiella; Q fever; cell lysis; purification; vacuole; infectivity; sonication; intracellular parasite
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Funding
- Intramural NIH HHS [Z01 AI000931-05] Funding Source: Medline
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Purification of the obligate intracellular bacterium Coxiella burnetii requires physical disruption of infected cells. Here we describe a gentle and safe digitonin lysis procedure to release C. burnetii from infected cells. The purity, yield, and infectivity of digitonin-prepped organisms are comparable to that of organisms purified using cell lysis by sonication. Published by Elsevier B.V.
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