Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 73, Issue 1, Pages 73-77Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2008.01.001
Keywords
quantitative PCR; DNA shuffling; real-time PCR; positive controls; standards; recA; Dehalococcoides
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We developed and tested a method to produce DNA standards and controls for quantitative PCR by designing and performing partial hybridization of long oligonucleotides before double stranded DNA fragments were synthesized and subsequently amplified by conventional PCR. This approach does not require any natural DNA template. Applications include the production of standards, which cannot be easily produced from DNA extracted from bacteria or plants. (C) 2008 Published by Elsevier B.V.
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