Journal
JOURNAL OF MEDICINAL CHEMISTRY
Volume 55, Issue 11, Pages 5425-5432Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jm300380a
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Funding
- California Tobacco-Related Disease Research Program [19FT-0105]
- NIH (NRSA) [1F32CA156833]
- Alexander von Humboldt foundation
- American Cancer Society [PF-10-015-01-CDD]
- National Institutes of Health [5T32GM007616, GM27681, GM51747]
- Ellison Medical Foundation [AG-SS-2256-09]
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To optimize the biological activity of pyrrole imidazole polyamide DNA-binding molecules, we characterized the aggregation propensity of these compounds through dynamic light scattering and fractional solubility analysis. Nearly all studied polyamides were found to form measurable particles 50-500 nm in size under biologically relevant conditions, while HPLC-based analyses revealed solubility trends in both core sequences and peripheral substituents that did not correlate with overall ionic charge. The solubility of both hairpin and cyclic polyamides was increased upon addition of carbohydrate solubilizing agents, in particular, 2-hydroxypropyl-beta-cyclodextrin (Hp beta CD). In mice, the use of Hp beta CD allowed for improved injection conditions and subsequent investigations of the availability of polyamides in mouse plasma to human cells. The results of these studies will influence the further design of Py-Im polyamides and facilitate their study in animal models.
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