Journal
JOURNAL OF MEDICINAL CHEMISTRY
Volume 53, Issue 6, Pages 2401-2408Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jm9014026
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Funding
- Fond di Ateneo per la Ricerca (PRA 2006, Universita di Messina)
- European Union
- METOXIA
- Academy of Sciences of the Czech Republic [AV0Z50520514, AV0Z40550506]
- Ministry of Education of the Czech Republic [1M0505]
- Czech Grant Foundation [GA203/09/0820]
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Following previous studies we herein report the exploration of the carbonic anhydrase (CA, EC 4.2.1.1) inhibitory effects and enzyme selectivity of a small class of 1-(cyclo)alkylisoquinolines containing a sulfonamide function considered a key feature for inhibiting CA. The results of enzymatic assays against human (h) CA isoforms, hCA I and hCA II (cytosolic, ubiquitous enzymes), hCA IX (transmembrane, tumor-associated), and hCA XIV (transmembrane), suggested that the presence of C-1 small substituents on isoquinoline scaffold controls both inhibitory potency and selectivity. Some derivatives showed potent hCA IX and hCA. XIV inhibitory effects at nanomolar concentrations as well as low affinity for the ubiquitous hCA II. Moreover, we report the X-ray crystal structure of one of these derivatives in complex with dominant human isoform II, thus confirming the sulfonamide zinc interactions. Finally, the results of docking experiments suggested the hypothetic interactions in the catalytic binding site for the most active and selective hCA IX and hCA XIV inhibitor.
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