Journal
JOURNAL OF MEDICAL PRIMATOLOGY
Volume 38, Issue 1, Pages 1-14Publisher
WILEY-BLACKWELL PUBLISHING, INC
DOI: 10.1111/j.1600-0684.2008.00299.x
Keywords
haploidentical animals; immunogenetics; Macaca fascicularis; selective breeding
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Funding
- UK Department of Health
- EUPRIM-NET
- NIAID [N01-AI-40088]
- NIH [1R21AI068488-01A2]
- MRC [G0601201, G9025730] Funding Source: UKRI
- Medical Research Council [G0601201, G9025730] Funding Source: researchfish
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Background Mauritian cynomolgus macaques have greatly restricted genetic diversity in the MHC region compared to other non-human primates; however, the frequency of common MHC haplotypes among captive-bred populations has not been reported. Methods Microsatellite PCR was used to determine MHC haplotype frequencies among captive macaques at a UK breeding facility. Allele-specific PCR and reference strand conformational analysis were used to determine the allele expression pro. le of a subset of animals. Results Haplotypes H3 (21%) and H1 (19%) were most common in the captive population of Mauritian cynomolgus macaques. Predicted alleles were detected by allele-specific PCR-SSP in 98% of animals. Allele expression profiles were similar in animals with identical haplotypes. Conclusions Mauritian cynomolgus macaques in the UK breeding facility have restricted MHC diversity comparable to a previously described population. Microsatellite-derived haplotypes are highly predictive of allele expression. A selective breeding program has been established to produce MHC-identical animals for biomedical research.
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