4.3 Article

Multilocus sequence typing of DNA from faecal specimens for the analysis of intra-familial transmission of Helicobacter pylori

Journal

JOURNAL OF MEDICAL MICROBIOLOGY
Volume 62, Issue -, Pages 761-765

Publisher

MICROBIOLOGY SOC
DOI: 10.1099/jmm.0.053140-0

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Funding

  1. Ministry of Health, Labor and Welfare, Japan
  2. Japan Society for the Promotion of Science [22590613, 23590518, 24593166]
  3. Waksman Foundation of Japan Inc.
  4. Wellcome Trust
  5. Grants-in-Aid for Scientific Research [22590613, 23590821, 23590518, 24593166] Funding Source: KAKEN

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This study used multilocus sequence typing (MLST) of total DNA extracted from faecal specimens to genotype Helicobacter pylori to analyse intra-familial transmission. Faecal DNA was extracted and amplified by nested PCR. The products were analysed by direct sequencing and the allele type was determined using an MLST website. Mother-to-child transmission was suspected in at least two of three families, and father-to-child transmission was suspected in one family.

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