Journal
JOURNAL OF MEDICAL MICROBIOLOGY
Volume 59, Issue 8, Pages 920-924Publisher
MICROBIOLOGY SOC
DOI: 10.1099/jmm.0.019752-0
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Funding
- Alberta Ingenuity Centre for Carbohydrate Science (AICCS)
- National Sciences and Engineering Council of Canada (NSERC)
- Canadian Institutes of Health Research (CIHR)
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Herein we describe a real-time quantitative PCR assay for evaluating the adherence of Clostridium difficile to differentiated human intestinal Caco-2 cells. Our investigations demonstrated that the method, employing the C. difficile-specific triose-phosphate isomerase gene, is as reliable but less time-consuming than counting c.f.u. We conclude that the method will be useful for evaluating the role of host cell adherence in the pathogenesis of C. difficile infection.
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