Journal
JOURNAL OF MEDICAL ENTOMOLOGY
Volume 49, Issue 4, Pages 939-941Publisher
ENTOMOLOGICAL SOC AMER
DOI: 10.1603/ME11287
Keywords
surveillance; mosquito pool; qRT-PCR; probe binding region; mutation
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Funding
- California Mosquito-borne Surveillance Program
- Mosquito and Vector Control Association of California
- NIH [AI55607, AI065359]
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A naturally occurring mutation was detected within the probe binding region targeting the envelope gene sequence of West Nile virus used in real-time polymerase chain reaction assays to test mosquito pools and other samples. A single C -> T transition 6nt from the 5' end of the 16mer in the envelope gene probe-binding region at genomic position 1,194 reduced assay sensitivity. The mutation first was detected in 2009 and persisted at a low prevalence into 2011. The mutation caused a 0.4% false negative error rate during 2011. These data emphasized the importance of confirmational testing and redundancy in surveillance systems relying on highly specific nucleic acid detection platforms.
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