Journal
JOURNAL OF MATERNAL-FETAL & NEONATAL MEDICINE
Volume 27, Issue 16, Pages 1656-1660Publisher
INFORMA HEALTHCARE
DOI: 10.3109/14767058.2013.871697
Keywords
Array-based comparative genomic hybridization; coelomic fluid; fetal DNA; QF-PCR; transabdominal coelocentesis
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Funding
- MIUR (Ministero Italiano dell'Universita e della Ricerca)
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This study reports a comparative analysis between results of transabdominal coelocentesis and traditional invasive procedure in order to assess the usefulness of coelocentesis as a source of fetal DNA for molecular and chromosomal analysis. A number of 28 women were included in the study. A successful sampling of coelomic fluid was obtained in 25 women by transabdominal procedure. A positive amplification of DNA with QF-PCR techniques was obtained in 90% of cases, while 10% of cases failed to reveal interpretable results. Although all samples were cultured, the growth rate was not sufficient to determine karyotypes within 2 weeks. Five samples were selected to be analyzed by array-based comparative genomic hybridization (a-CGH) but the interpretation of these results was difficult and ambiguous. Our results suggest that transabdominal coelocentesis is suitable for the detection of single DNA variation and for QF-PCR analysis, while further experiments are needed to develop optimized protocols for traditional karyotyping and array-analysis.
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