Journal
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
Volume 21, Issue 2, Pages 741-751Publisher
SPRINGER
DOI: 10.1007/s10856-009-3871-5
Keywords
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Funding
- National Natural Science Foundation of China [30772186]
- Youth Science and Research Foundation of the Third Military Medical University [XG2005D127]
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To construct a novel scaffold for nucleus pulposus (NP) tissue engineering, The porous type II collagen (CII)/hyaluronate (HyA)-chondroitin-6-sulfate (6-CS) scaffold was prepared using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) and N-hydroxysuccinimide (NHS) cross-linking system. The physico-chemical properties and biocompatibility of CII/HyA-CS scaffolds were evaluated. The results suggested CII/HyA-CS scaffolds have a highly porous structure (porosity: 94.8 +/- A 1.5%), high water-binding capacity (79.2 +/- A 2.8%) and significantly improved mechanical stability by EDC/NHS crosslinking (denaturation temperature: 74.6 +/- A 1.8 and 58.1 +/- A 2.6A degrees C, respectively, for the crosslinked scaffolds and the non-crosslinked; collagenase degradation rate: 39.5 +/- A 3.4 and 63.5 +/- A 2.0%, respectively, for the crosslinked scaffolds and the non-crosslinked). The CII/HyA-CS scaffolds also showed satisfactory cytocompatibility and histocompatibility as well as low immunogenicity. These results indicate CII/HyA-CS scaffolds may be an alternative material for NP tissue engineering due to the similarity of its composition and physico-chemical properties to those of the extracellular matrices (ECM) of native NP.
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