Journal
JOURNAL OF MATERIALS CHEMISTRY
Volume 21, Issue 33, Pages 12358-12362Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c1jm11436k
Keywords
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Funding
- Singapore A*Star SERC [072 101 0020]
- China NNSF [50902071, 61076067, 61006007]
- China 973 Program [2009CB930601]
- Science Foundation of Nanjing University of Posts and Telecommunications [NY208058]
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Graphene, which is a recently discovered single-atom-thick planar sheet of carbon atoms perfectly arranged in a honeycomb lattice, has great potential in biosensing owing to its extraordinary electrical, physical, and optical properties. In this work, we demonstrate a graphene based biosensor to electrically detect E. coli bacteria with high sensitivity and specificity. The large-sized graphene film was grown by chemical vapor deposition and functionalized with anti-E. coli antibodies and passivation layer. Significant conductance increase of the graphene device was observed after exposure to E. coli bacteria at a concentration as low as 10 cfu/mL, while no significant response was triggered by high concentration of the another bacterial strain. In addition, this biosensor was employed to detect the glucose induced metabolic activities of the bound E. coli bacteria in real time. This simple, fast, sensitive, and label-free nanoelectronic biosensor, in principle, could serve as a high throughput platform for detection of any pathogenic bacteria, and for functional studies or screening of antibacterial drugs.
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