Journal
JOURNAL OF MATERIALS CHEMISTRY
Volume 21, Issue 14, Pages 5319-5325Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0jm03441j
Keywords
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Funding
- National Institute of Neurological Disorders and Stroke [NS058161-01]
- Pacific Northwest National Laboratory (PNNL)
- U.S. Department of Energy (DOE) [DE-AC05-76RL01830]
- DOE's office of Biological and Environmental Research located at PNNL
- National Natural Science Foundation of China [20975060, 21075047]
- National Basic Research Program of China [2007CB310500]
- Program for Chenguang Young Scientist for Wuhan [200950431184]
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A nanohybrid of gold nanoparticles (Au NPs) and chemically reduced graphene oxide nanosheets (cr-Gs) was synthesized by in situ growth of Au NPs on the surface of graphene nanosheets in the presence of poly(diallyldimethylammonium chloride) (PDDA), which not only improved the dispersion of Au NPs but also stabilized cholinesterase with high activity and loading efficiency. The obtained nanohybrid was characterized by TEM, XRD, XPS, and electrochemistry. Then an enzyme nanoassembly (AChE/Au NPs/cr-Gs) was prepared by self-assembling acetylcholinesterase (AChE) on Au NP/cr-Gs nanohybrid. An electrochemical sensor based on AChE/Au NPs/cr-Gs was further developed for ultrasensitive detection of organophosphate pesticide. The results demonstrate that the developed approach provides a promising strategy to improve the sensitivity and enzyme activity of electrochemical biosensors.
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