Journal
JOURNAL OF MATERIALS CHEMISTRY
Volume 21, Issue 47, Pages 18877-18886Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c1jm12078f
Keywords
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Funding
- Air Force Office of Scientific Research
- MURI
- DOE Bioenergy Science Center (BESC)
- USDA Biodesign and Bioprocess Center
- China National Special Fund for Key Laboratories [2060204]
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While most in vitro synthetic biology projects are usually used for the purposes of basic science research or the formation of high-value products, cell-free synthetic pathway biotransformation (SyPaB), which can implement complicated biochemical reactions by the in vitro assembly of numerous enzymes and coenzymes, would be used for low-cost biomanufacturing. In this article, we present bottom-up design principles for SyPaB from basic building blocks (enzymes and/or immobilized enzymes) to basic modules, such as NAD(P) H regeneration, NAD(P) H consumption, ATP regeneration, and extra ATP removal. A combination of thermostable enzymes (called thermoenzymes) with immobilization on solid supports, especially nano-materials and/or electrodes, would greatly prolong enzyme lifetime, enhance mass transfer, and facilitate product/biocatalyst separation. With developments in stable building blocks and modules (called biocatalytic modules), SyPaB has the potential to become a low-cost biomanufacturing platform for biofuels production and even biological CO2 fixation.
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