Facile one-pot synthesis of bimodal mesoporous carbon nitride and its function as a lipase immobilization support

Bimodal mesoporous carbon nitride is synthesized using Triton X-100 as a template and melamine and glutaraldehyde as precursors through polymerization and carbonization. The structures and surface chemical properties of carbon nitrides are characterized and compared by nitrogen adsorption isotherms, TG, FT-IR spectra and SEM images. The results show that the mesopore sizes in the carbon nitride are centered at 3.8 nm and 10-40 nm. The pyrolysis and decomposition of the polymer to form the condensation cross-linking ring structures takes place at 350-700 degrees C. The nitrogen atom in the carbon nitride exists in C-N and N-H states. As a Candida rugosa lipase immobilization support, the mesoporous carbon nitride displays a higher amount of immobilization and improves the thermal stability of Candida rugosa lipase, which still displays higher catalytic activity after 4 runs.