4.3 Article

Barstar:barnase - a versatile platform for colloidal diamond bioconjugation

Journal

JOURNAL OF MATERIALS CHEMISTRY
Volume 21, Issue 1, Pages 65-68

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c0jm02819c

Keywords

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Funding

  1. Macquarie University [1136900]
  2. Russian Academy of Sciences
  3. Russian Foundation of Basic Research [09-04-01201]
  4. Russian Federal Agency for Science and Innovation

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We report on a bioconjugation platform based on a high-affinity protein pair, barstar: barnase (Bs:Bn), that provides a modular design toolkit capable of strong (covalent) and versatile assembly of bio/nanocomplexes. Luminescent nanodiamonds (140 nm) serve as the docking station to synthesize Bs: Bn bioconjugates which are characterized and utilized in several applications, including cell transfection.

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