Journal
JOURNAL OF MATERIALS CHEMISTRY
Volume 21, Issue 14, Pages 5352-5359Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0jm03010d
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Funding
- National Natural Science Foundation of China [20773040, 50702022]
- Research Fund for the Doctoral Program (New Young Teacher) of Higher Education [20070561005]
- Fundamental Research Funds for the Central Universities [SCUT2009ZZ0010, SCUT2009ZM0015]
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Nanoplated lanthanum-substituted bismuth titanate (Bi(3.25)La(0.75)Ti(3)O(12), BLTO) microspheres constructed with tens of BLTO nanoplates were synthesized by a sol-gel hydrothermal method. Using nanoplated BLTO microspheres, a novel third-generation H(2)O(2) biosensor was fabricated with loading of myoglobin (Mb) and chitosan (Chi). Scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), and X-ray diffraction (XRD) measurements reveal that partial Bi ions of bismuth titanate (BTO, Bi(4)Ti(3)O(12)) are successfully substituted with La ions by the sol-gel hydrothermal method. UV-visible (UV-vis) and Fourier-transform infrared (FTIR) spectra show that Mb encapsulated in the Mb-Chi-BLTO film can retain its bioactivity well. Comparative experiments witness that the Mb-Chi-BLTO biosensor, compared with the Mb-Chi-BTO biosensor, not only has enhanced direct electron-transfer capacity (e. g., stronger redox peak currents (approximately 3-fold) and a larger heterogeneous electron-transfer rate constant of 12.8 +/- 3.3 s(-1)), but also exhibits a wider linear response to H2O2 in the concentration range of 2-490 mu M, higher sensitivity (88 mA cm(-2) M(-1)), a lower Michaelis-Menten constant (0.55 mM) and detection limit (0.14 mu M), a shorter response time (2.8 s), and better reproducibility and stability. These results imply that La doping greatly improves electrochemical and electrocatalytic properties of the Mb-Chi-BLTO biosensor, which will open up a new idea for the design of third-generation electrochemical biosensors, and the BLTO-based biosensors are also expected to find potential applications in many areas such as clinical diagnosis and food and environmental detection.
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