Journal
JOURNAL OF MATERIALS CHEMISTRY
Volume 20, Issue 33, Pages 6935-6941Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0jm00506a
Keywords
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Funding
- 863 Program [2009AA03Z313]
- National Natural Science Foundation of China [30772007, 30970784]
- National Grand Program on Key Infectious Disease Control [2008ZX10001-015-10]
- National Key Basic Research Program of China [2009CB930200]
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pH-dependent temperature-sensitive poly(epsilon-caprolactone)-graft-poly(2-(dimethylamino) ethyl methacrylate) (PCL-g-PDMAEMA), a kind of degradable, amphiphilic, cationic copolymer, was synthesized. PCL-g-PDMAEMA was self-assembled into core-shell nanoparticles with an ultralow critical association concentration at about 8.1 x 10(-4) g L(-1). It was found that PCL-g-PDMAEMA nanoparticles were able to simultaneously entrap hydrophobic paclitaxel and load DNA. Hydrophobic drug paclitaxel, loaded by PCL-g-PDMAEMA NPs, could be released faster in an acidic environment than in a neutral environment, and PCL-g-PDMAEMA NPs showed a comparable in vitro gene transfection efficiency to Lipofectamine 2000. In addition, the gene transfection efficiency was enhanced by the addition of 5% serum. Besides, confocal microscopic measurements indicated that PCL-g-PDMAEMA nanoparticles/DNA polyplexes could escape from the endosome and release the payloads effectively in cytoplasm. These results suggest PCL-g-PDMAEMA has great potential for achieving the synergistic effect of drug and gene therapies in vivo.
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