Journal
JOURNAL OF MASS SPECTROMETRY
Volume 48, Issue 7, Pages 768-773Publisher
WILEY
DOI: 10.1002/jms.3221
Keywords
doxorubicin; prodrug; tissue extraction; LC-MS; MS
Funding
- National Cancer Institute [5 U54 CA119335, T32 CA121938]
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The localized conversion of inactive doxorubicin prodrug chemotherapeutics to pharmacalogically active forms is difficult to quantify in mouse tumor models because it occurs only in small regions of tissue. The tumor tissue extraction protocol and LC-MS/MS analysis method described here were optimized to obtain a detection limit of 7.8pg for the activated doxorubicin and 0.36ng for the doxorubicin prodrug. This method can be useful for determining the biodistribution and activation efficiency for many different doxorubicin prodrugs. It can also be used for quantification of doxorubicin from tumor models that have poor vascularization resulting in low tissue accumulation. Copyright (c) 2013 John Wiley & Sons, Ltd.
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