Journal
JOURNAL OF MASS SPECTROMETRY
Volume 45, Issue 5, Pages 545-552Publisher
WILEY
DOI: 10.1002/jms.1740
Keywords
silica gel; pore structure; fullerene; MALDI; low-molecular-weight analytes
Funding
- Austrian Science Foundation (EWE)
- Vienna, Austria [021]
- Genome Research in Austria (GEN-AU)
- Federal Ministry for Education, Science and Culture, Vienna, Austria
- West Austrian Initiative for Nano Networking (WINN)
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In spite of the growing acceptance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the analysis of a wide variety of compounds, including polymers and proteins, its use in analyzing low-molecular-weight molecules (<1000 m/z) is still limited. This is mainly due to the interference of matrix molecules in the low-mass range. Here the derivatized fullerenes covalently bound to silica particles with different pore sizes are applied as thin layer for laser desorption/ionization (LDI) mass spectrometric analysis. Thus, an interference of intrinsic matrix ions can be eliminated or minimized in comparison with the state-of-the-art weak organic acid matrices. The desorption/ionization ability of the developed fullerene-silica materials depends on the applied laser power, sample preparation and pore size of the silica particles. Thus, fullerene-silica serves as an LDI support for mass spectrometric analysis of molecules (<1500 Da). The performance of the fullerene-silica is demonstrated by the mass analysis of variety of small molecules such as carbohydrates, amino acids, peptides, phospholipids and drugs. Copyright (C) 2010 John Wiley & Sons, Ltd.
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