Journal
JOURNAL OF MASS SPECTROMETRY
Volume 45, Issue 1, Pages 97-103Publisher
JOHN WILEY & SONS LTD
DOI: 10.1002/jms.1697
Keywords
MALDI tissue imaging; MALDI profiling; cocaine addiction; secretoneurin; neuropeptides
Funding
- USPHS [DA015369, DA015851]
- NATIONAL INSTITUTE ON DRUG ABUSE [R01DA015851, R01DA003906, P50DA015369] Funding Source: NIH RePORTER
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Proteins in the nucleus accumbens mediate many cocaine-induced behaviors. In an effort to measure changes in nucleus accumbens protein expression as potential biomarkers for addiction, coronal tissue sections were obtained from rats that developed behavioral sensitization after daily administration of cocaine, or from daily saline-treated controls. The tissue sections were subjected to matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) profiling and tissue imaging. For profiling experiments, brain sections were manually spotted with matrix over the nucleus accumbens, a brain region known to regulate cocaine sensitization. Summed mass spectra (110 000 laser shots, grid) were acquired and spectra were aligned to reference peaks. Using bioinformatics tools, eight spectral features were found to be altered by cocaine treatment. Based on additional sequencing experiments with MALDI tandem MS and database searches of measured masses, secretoneurin (m/z 3653) was identified as having an increased expression. In addition, the distribution of m/z 3653 in the nucleus accumbens was determined by MALDI tissue imaging, and the increased expression of its precursor protein, secretogranin 11, was verified by immunoblotting. Copyright (C) 2009 John Wiley & Sons, Ltd.
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