Journal
PARASITOLOGY RESEARCH
Volume 114, Issue 12, Pages 4487-4492Publisher
SPRINGER
DOI: 10.1007/s00436-015-4692-8
Keywords
Aedes vexans; Mosquito vectors; Dirofilaria repens; Dirofilaria immitis; Slovakia
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Funding
- VEGA - European Regional Development Fund [1/0080/15, 0.7]
- National Referential Laboratory for Pesticides of the University of Veterinary Medicine - European Regional Development Fund
- project Centre of Excellence for Parasitology (ITMS) - European Regional Development Fund [26220120022]
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This paper follows the study from 2013 focused on the molecular screening of mosquitoes as vectors of Dirofilaria spp. which provided the information on Aedes vexans as a potential vector of Dirofilaria repens in Slovakia. Current entomological and molecular research indicates that Ae. vexans can participate also in the transmission of Dirofilaria immitis within the region. Using the standard PCR method, we examined 10,500 mosquitoes (Ae. vexans, Ae. rossicus, Anopheles maculipennis s.l., Ochlerotatus sticticus, Ochlerotatus cantans, Ochlerotatus caspius, Culex pipiens/Culex torrentium, Coquillettidia richiardii), collected using CO2-baited traps at six locations in the Eastern Slovakia. Out of 105 pools, 6 pools of mosquitoes Ae. vexans were positive for D. repens DNA (minimum infective rate in Ae. vexas was 6:6.900, i.e. 0.8 per 1.000 mosquitoes), within which 4 were concurrently positive for D. immitis (minimum infective rate in Ae. vexans was 4:6.900 i.e. 0.5 per 1.000 mosquitoes).
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