Journal
JOURNAL OF MASS SPECTROMETRY
Volume 43, Issue 9, Pages 1235-1254Publisher
WILEY
DOI: 10.1002/jms.1400
Keywords
triple quadrupole mass spectrometry (QqQ); gas chromatography; QuEChERS extraction; multiresidue analysis; food commodities; pesticide
Funding
- Ministerio de Educacion y Ciencia of Spain (MEC-FEDER) [CTQ2005-03654/BQU, AP2005-3800]
- Consejeria de Innovacion Ciencia y Empresa de la Junta de Andalucia [P05-FQM-0202]
Ask authors/readers for more resources
A new multiresidue method has been developed and validated for the determination of more than 140 pesticide residues in cucumber and orange by gas chromatography coupled to triple quadrupole mass spectrometry (GC-QqQ-MS/MS) in a single run of 25.50 min. The triple quadrupole (QqQ) analyzer simultaneously operated in the selected reaction monitoring (SRM) and selected ion monitoring (SIM) modes, acquiring two or three transitions per compound. Samples were extracted by the application of a single-phase extraction of 10 g of sample with acetonitrile containing 1% of acetic acid, followed by a liquid-liquid partition formed by the addition of 4 g Of MgSO4 and 1 g of NaOAc. A dispersive solid-phase extraction (D-SPE) with primary secondary amine (PSA) was applied to clean up the extracts. A final concentration step was included in order to increase sensitivity in the instrumental analysis. The method was properly validated in each matrix in a wide dynamic range (10-400 mu g kg(-1)): this work relies on a new quantification strategy by the use of two calibration curves to increase the dynamic range, which permitted reduction of sample dilutions and increase in sample throughput. Recovery was studied at three concentration levels (11.5, 50.0, and 150.0 mu g kg(-1)), yielding values in the range 70-110% with precision values, expressed as relative standard deviation (RSD), lower than 20 and 25% for the intraday and interday precision, respectively. Limits of quantification (LOQs) were established at 10 mu g kg(-1), the lowest maximum residue level (MRL) value set by the European Union in vegetables. The method was successfully applied to the analysis of pesticide residues in real samples from the southeastern Spain. Copyright (C) 2008 John Wiley & Sons, Ltd.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available