Journal
PARASITOLOGY INTERNATIONAL
Volume 64, Issue 3, Pages 290-294Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.parint.2014.09.013
Keywords
Liver stage; Malaria; Peroxiredoxin; Plasmodium berghei; Thioredoxin peroxidase
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Funding
- JSPS [255843, 23390098]
- Grants-in-Aid for Scientific Research [13J05843, 23390098] Funding Source: KAKEN
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Phenotypic observation of thioredoxin peroxidase-1 (TPx-1) gene-disrupted Plasmodium berghei (TPx-1 KO) in the liver-stage was performed with an in vitro infection system in order to investigate defective liver-stage development in a mouse infection model. Indirect immunofluorescence microscopy assay with anti-circumsporozoite protein antibody revealed that in the liver schizont stage, TPx-1 1(0 parasite cells were significantly smaller than cells of the wild-type parent strain (WT). Indirect immunofluorescence microscopy assay with anti-merozoite surface protein-1 antibody, which was used to evaluate late schizont-stage development, indicated that TPx-1 KO schizont development was similar to WT strain development towards the merozoite-forming stage (mature schizont). However, fewer merozoites were produced in the mature TPx-1 KO schizont than in the mature WT schizont. Taken together, the results suggest that TPx-1 may be involved in merozoite formation during liver schizont development. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
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