Journal
JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS
Volume 323, Issue 3-4, Pages 272-278Publisher
ELSEVIER
DOI: 10.1016/j.jmmm.2010.09.014
Keywords
Biosensing; Diagnostics; Magnetic beads; Magnetic rotation; Biotin; Streptavidin; Sandwich Immunoassay
Funding
- Department of Homeland Security (DHS)
- U.S. Department of Energy (DOE)
- Oak Ridge Associated Universities (ORAU) [DE-AC05-060R23100]
- NIH [UL1RR024986, R21EB009550]
- [NSF/DMR 0455330]
Ask authors/readers for more resources
This paper presents a novel application of magnetic particles for biosensing, called label-acquired magnetorotation (LAM). This method is based on a combination of the traditional sandwich assay format with the asynchronous magnetic bead rotation (AMBR) method. In label-acquired magnetorotation, an analyte facilitates the binding of a magnetic label bead to a nonmagnetic solid phase sphere, forming a sandwich complex. The sandwich complex is then placed in a rotating magnetic field, where the rotational frequency of the sandwich complex is a function of the amount of analyte attached to the surface of the sphere. Here, we use streptavidin-coated beads and biotin-coated particles as analyte mimics, to be replaced by proteins and other biological targets in future work. We show this sensing method to have a dynamic range of two orders of magnitude. (C) 2010 Elsevier B.V. Allrightsreserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available