Journal
JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS
Volume 320, Issue 15, Pages 2049-2055Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jmmm.2008.03.015
Keywords
Au-Fe oxide nanoparticle; magnetic nanoparticle; magnetic separation; biological application
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Gold-coated magnetic nanoparticles were synthesized with size ranging from 15 to 40nm using sodium citrates as the reducing agent. Oxidized magnetites (Fe(3)O(4)) fabricated by co-precipitation of Fe(2+) and Fe(3+) in strong alkaline solution were used as magnetic cores. The structures of gold (Au) shell and magnetic core (Au-Fe) were studied by transmission electron microscopy (TEM) image and energy dispersive spectroscopy (EDS) spectrum. Results from high-resolution X-ray diffraction (HR XRD) show that the Au-Fe oxide nanoparticles have a face-centered cubic shape with the crystalline faces of {111}. The Au-coated magnetic nanoparticles exhibited a surface plasmon resonance peak at 528 nm. The nanoparticles are well dispersed in distilled water. A 3000G permanent magnet was successfully used for the separation of the functionalized nanoparticles. Magnetic properties of the nanoparticles were determined by magnetic force microscope (MFM) in nanometric resolution and vibrating sample magnetometer (VSM). Magnetic separation of biological molecules using Au-coated magnetic oxide composite nanoparticles was examined after attachment of protein immunoglobulin G (IgG) through electrostatic interactions. Using this method, separation was achieved with a maximum yield of 35% at an IgG concentration of 400 ng/ml. (c) 2008 Elsevier B.V. All rights reserved.
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