Analytical model for the magnetophoretic capture of magnetic microspheres in microfluidic devices

Magnetic microspheres are used as mobile substrates in micro-total-analysis systems (mu TAS), since the particles can be selectively functionalized to attach different bioconjugates and can be precisely manipulated using external magnetic field gradients. A large number of MEMS-based bio-analytical devices employ magnetophoretic separation as an important step during their operation. An analytical technique is proposed in this paper that describes the magnetophoretic transport of magnetic microspheres under an imposed magnetic field when there is a pressure-driven or electroosmotic flow through a microchannel. Successful magnetophoretic capture occurs if the strength of the field-inducing magnetic dipole exceeds a critical value, or if the particles are larger than a critical size. The magnetophoretic separator performance is characterized in terms of capture efficiency. The analysis shows that the capture efficiency is a function of two independent non-dimensional parameters, lambda and gamma that in turn involve all the physical design and operating parameters of the microfluidic separator, e. g., the dipole strength, particle size and susceptibility, fluid viscosity and velocity, channel height, and the separation of the dipole. Parametric plots of capture efficiency as function of lambda and gamma helps in choosing the right design and operation parameter of a practical microfluidic separator for a target level of performance. (c) 2007 Elsevier B. V. All rights reserved.