Journal
JOURNAL OF MAGNETIC RESONANCE
Volume 244, Issue -, Pages 85-89Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jmr.2014.04.017
Keywords
Solid-state NMR; Polarization transfer; Magic-angle spinning; 14N; (CP)-C-RESPIRATION
Funding
- Danish Strategic Research Council
- Danish National Research Foundation [DNRF59]
- European Commission [Bio-NMR 261863]
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Polarization transfer between spin-1/2 nuclei and quadrupolar spin-1 nuclei such as N-14 in solid-state NMR is severely challenged by the typical presence of large quadrupole coupling interactions. This has effectively prevented the use of the abundant N-14 spin as a probe to structural information and its use as an element in multi-dimensional solid-state NMR correlation experiments for assignment and structural characterization. In turn, this has been a contributing factor to the extensive use of isotope labeling in biological solid-state NMR, where N-14 is replaced with N-15. The alternative strategy of using the abundant N-14 spins calls for methods enabling efficient polarization transfer between N-14 and its binding partners. This work demonstrates that the recently introduced (RESPIRATION) CP transfer method can be optimized to achieve efficient H-1 <-> N-14 polarization transfer under magic angle spinning conditions. The method is demonstrated numerically and experimentally on powder samples of NH4NO3 and L-alanine. (C) 2014 Elsevier Inc. All rights reserved.
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