Journal
JOURNAL OF MAGNETIC RESONANCE
Volume 214, Issue -, Pages 329-334Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jmr.2011.10.019
Keywords
NMR; Protein; IDP; Sequential resonance assignment; HADAMAC
Funding
- European Commission [261862, 264257, 231082]
- ARC
- CEA
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An experiment, iHADAMAC, is presented that yields information on the amino-acid type of individual residues in a protein by editing the H-1-N-15 correlations into seven different 2D spectra, each corresponding to a different class of amino-acid types. Amino-acid type discrimination is realized via a Hadamard encoding scheme based on four different spin manipulations as recently introduced in the context of the sequential HADAMAC experiment. Both sequential and intra-residue HADAMAC experiments yield highly complementary information that greatly facilitate resonance assignment of proteins with high frequency degeneracy, as demonstrated here for a 188-residue intrinsically disordered protein fragment of the hepatitis C virus protein NS5A. (C) 2011 Elsevier Inc. All rights reserved.
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