Journal
JOURNAL OF MAGNETIC RESONANCE
Volume 203, Issue 1, Pages 52-56Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jmr.2009.11.020
Keywords
Screening; Ligand binding; Dissolution DNP
Funding
- GE
Ask authors/readers for more resources
NMR spectroscopy is an established, versatile technique for the detection of molecular interactions, even when these interactions are weak Signal enhancement by several orders of magnitude through dynamic nuclear polarization alleviates several practical limitations of NMR-based interaction studies This enhanced non-equilibrium polarization contributes sensitivity for the detection of molecular interactions in a single NMR transient We show that direct C-13 NMR ligand binding studies at natural isotopic abundance of C-13 gets feasible in this way Resultant screens are easy to interpret and can be performed at C-13 concentrations below mu M In addition to such ligand-detected studies Of molecular interaction, ligand binding can be assessed and quantified with enzymatic assays that employ hyperpolarized substrates at varying enzyme inhibitor concentrations The physical labeling of nuclear spins by hyperpolarization thus provides the opportunity to devise fast novel in vitro experiments with low material requirement and without the need for synthetic modifications of target or ligands (C) 2009 Elsevier Inc All rights reserved
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available