Journal
JOURNAL OF MAGNETIC RESONANCE
Volume 207, Issue 1, Pages 164-167Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jmr.2010.08.002
Keywords
PELDOR; DEER; Relaxation; EPR; T-m; Deuteration
Funding
- EPSRC [EP/F030401/1]
- BBSRC [BB/E022286/1]
- Wellcome Trust
- Biotechnology and Biological Sciences Research Council [BB/E022286/1] Funding Source: researchfish
- BBSRC [BB/E022286/1] Funding Source: UKRI
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One of the major problems facing distance determination by pulsed EPR, on spin-labeled proteins, has been the short relaxation time T-m. Solvent deuteration has previously been used to slow relaxation and so extend the range of distance measurement and sensitivity. We demonstrate here that deuteration of the underlying protein, as well as the solvent, extends the T-m to a considerable degree. Longer T-m gives greatly enhanced sensitivity, much extended distance measurement, more reliable distance distribution calculation and better baseline correction. (C) 2010 Elsevier Inc. All rights reserved.
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