Journal
JOURNAL OF MAGNETIC RESONANCE
Volume 202, Issue 2, Pages 140-146Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jmr.2009.10.008
Keywords
Bioreactor; In-cell nuclear magnetic resonance (NMR)
Funding
- NIEHS NIH HHS [R21 ES010774-01, R21 ES010774-02] Funding Source: Medline
- NIH HHS [DP1 OD000783-03S1, DP1 OD000783-02, DP1 OD000783-03, DP1 OD000783-01, DP1 OD000783-04, DP1 OD000783] Funding Source: Medline
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The inside of the cell is a complex environment that is difficult to simulate when studying proteins and other molecules in vitro. We have developed a device and system that provides a controlled environment for nuclear magnetic resonance (NMR) experiments involving living cells. Our device comprises two main parts, an NMR detection region and a circulation system. The flow of medium from the bottom of the device pushes alginate encapsulated cells into the circulation chamber. In the chamber, the exchange of oxygen and nutrients occurs between the media and the encapsulated cells. When the media flow is stopped, the encapsulated cells fall back into the NMR detection region, and spectra can be acquired. We have utilized the bioreactor to study the expression of the natively disordered protein alpha-synuclein, inside Escherichia coli cells. (C) 2009 Elsevier Inc. All rights reserved.
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