Journal
JOURNAL OF MAGNETIC RESONANCE
Volume 191, Issue 2, Pages 202-218Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jmr.2007.11.023
Keywords
DEER; EPR spectroscopy; structure elucidation; dipolar interactions; spin labels
Funding
- Engineering and Physical Sciences Research Council [EP/D048559/1] Funding Source: researchfish
- EPSRC [EP/D048559/1] Funding Source: UKRI
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Inter-spin distances between 1 nm and 4.5 nm are measured by continuous wave (CW) and pulsed electron paramagnetic resonance (EPR) methods for a series of nitroxide-spin-labelled peptides. The upper distance limit for measuring dipolar coupling by the broadening of the CW spectrum and the lower distance limit for the present optimally-adjusted double electron electron resonance (DEER) set-up are determined and found to be both around 1.6-1.9 nm. The methods for determining distances and corresponding distributions from CW spectral line broadening are reviewed and further developed. Also, the work shows that a correction factor is required for the analysis of inter-spin distances below approximately 2 nm for DEER measurements and this is calculated using the density matrix formalism. (c) 2007 Elsevier Inc. All rights reserved.
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