Journal
JOURNAL OF MACROMOLECULAR SCIENCE PART A-PURE AND APPLIED CHEMISTRY
Volume 48, Issue 6, Pages 447-453Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/10601325.2011.573330
Keywords
Mesostructured cellular foam; immobilization; laccase; decolorization; synthetic dyes
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Funding
- National Natural Science Fundation of China [21075025]
- Natural Science Fundation of Heilongjiang Province [B200910]
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Laccase was immobilized on mesostructured cellular foam (MCF), a kind of mesoporous silica with large pore size by adsorption-cross linking method. The effects of immobilization time, temperature, pH, amount of enzyme and content of glutaraldehyde on the immobilization were optimized. The activities and stabilities towards pH and temperature of the immobilized enzyme were studied, and significantly improved enzymatic properties and good operational stability were obtained for the immobilized laccase. Dye decolorization tests showed that the immobilized enzyme could decolorize Alizarin Red and Indigo Blue solution fast and efficiently in the presence of ABTS.
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