Application of Box-Behnken design in the optimization of new peroxyoxalate-H2O2 chemiluminescence system using furan derivatives as blue activators

The non-commercially synthesized and purified furan derivatives are of great interest as fluorescent emitters for peroxyoxalate chemiluminescence (PO-CL). The reaction of oxalic ester such as bis-(2,4,6-trichloro-phenyl) oxalate (TCPO) with H2O2 can lead to the excitation of an appropriate activator via the formation of a 1,2-dioxetanedione intermediate. In this study, two furan derivatives were used as activators which produce a blue light in the chemiluminescence systems. In the following, the Box-Behnken design matrix and response surface methodology (RSM) have been applied to design the experiments to study the relationship between the chemiluminescence intensities and the three most important operating variables such as sodium salicylate (5 x 10(-4)-5 x 10(-3) M), furan (10(-5)-10(-3) M) and hydrogen peroxide (10(-3)-10(-1) M) concentrations, and also to evaluate the interactive effects of these variables on the CL emission of TCPO system. Totally 15 experiments were conducted in the present study towards the construction of a quadratic model. Independent variables of sodium salicylate, hydrogen peroxide and furan concentration have the significance value of p=0.05, in both the systems, which explicitly shows the importance of these variables in the CL emission of TCPO. Values of Prob > F less than 0.0001 indicated that the model terms are significant for the CL emission of TCPO. Moreover, the regression equation coefficients were calculated and the data fitted to a second-order polynomial equation for CL emission of TCPO. (C)2012 Elsevier B.V. All rights reserved.