Excited state interaction between Hydrochlorothiazide and europium ion in PMMA polymer and its application as optical sensor for Hydrochlorothiazide in tablet and serum samples

This paper reports on a facile technique combined with a simple, sensitive and selective spectrofluorimetric method for the determination of hydrochlorothiazide. In methanol, at pH 8.3 and lambda(ex) = 340, hydrochlorothiazide can remarkably enhance the luminescence intensity of the Eu3+ ion doped in polymethylmethacrylate polymer (PMMA) matrix. This could be due to the energy transfer from hydrochlorothiazide to Eu3+ in the excited stated. At the optimized experimental conditions, the enhancement of the characteristic emission band (617 nm) of Eu3+ ion doped PMMA is directly proportional to the concentration of hydrochlorothiazide with a dynamic range of 5 x 10(-8)- 1.0 x 10(-5) mol L-1 and detection limit of 8.0 x 10(-9) mol L-1. Application of the suggested method was successfully applied to the determination of hydrochlorothiazide in pharmaceutical preparations and human serum samples, with high percentage of recovery, good accuracy and precision. (c) 2012 Elsevier B.V. All rights reserved.