Journal
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
Volume 38, Issue 8, Pages 898-903Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/10826076.2014.991869
Keywords
SPE; egg yolk; neutral lipids; phospholipids; charged aerosol detector (CAD); HPLC
Funding
- National Science Center (NCN) [NN 209 137840]
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In this paper the methods of different lipid classes analysis using high performance liquid chromatography (HPLC) with charged aerosol detector (CAD) are presented. The methods allowed base-line separation of all investigated egg yolk phospholipids (phosphatidylcholine, PC; phosphatidylethanolamine, PE; sphingomyelin, SM; and their lyso forms) and neutral lipid classes (triacylglycerol, TAG; cholesterol, Chol). Total lipids extraction was carried out by the Folch method. The polar and neutral lipid classes were separated by using normal-phase (silica) SPE cartridges. Two different HPLC methodologies (one for polar and one for neutral lipids) for the separation and quantification of lipid classes on a diol column were applied. The methods were optimized and the response and sensitivity of CAD was studied. In terms of linearity, precision, and sensitivity the methods were validated.
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