Journal
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
Volume 37, Issue 12, Pages 1771-1784Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/10826076.2013.809545
Keywords
simultaneous determination; quantification; pogostone; HPLC-DAD; flavonoids; Pogostemon cablin
Funding
- Macau Science and Technology Development Fund [013/2008/A1]
- University of Macau [RG087/07-08S/ZQW/ICMS]
- Ministry of Science and Technology of China [2013BAI11B05, 2013DFM30080]
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A high performance liquid chromatography (HPLC) method was developed and validated for simultaneous quantification of nine major components including volatile and nonvolatile ingredients in Pogostemon cablin (Blanco) Benth. The analysis was performed on a Cosmosil 5 C18-AR-II column (4.6mm-250mm, 5mm) with 0.2% formic acid in water-acetonitrile gradient elution. The method was validated in terms of linearity, sensitivity, precision, stability, and accuracy. It was found that the calibration curves for all analytes showed good linearity (R2>0.9991) within the test ranges. The overall limit of detection (LOD) and limit of quantification (LOQ) ere less than 36.32 and 122.09ng. The relative standard deviations (RSDs) for intra-day and inter-day repeatability were no more than 3.23% and 4.85%, respectively. The sample was stable for at least 48hr. The spike recoveries of nine components were 96-106%. The established method was successfully applied to determine nine components in thirteen samples from different locations. The results showed that the newly developed HPLC-DAD method was sensitive, precise, and accurate and could be used for quality control of P. cablin.
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