SIMULTANEOUS DETERMINATION OF EIGHT FLAVONOIDS AND POGOSTONE IN POGOSTEMON CABLIN BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

A high performance liquid chromatography (HPLC) method was developed and validated for simultaneous quantification of nine major components including volatile and nonvolatile ingredients in Pogostemon cablin (Blanco) Benth. The analysis was performed on a Cosmosil 5 C18-AR-II column (4.6mm-250mm, 5mm) with 0.2% formic acid in water-acetonitrile gradient elution. The method was validated in terms of linearity, sensitivity, precision, stability, and accuracy. It was found that the calibration curves for all analytes showed good linearity (R2>0.9991) within the test ranges. The overall limit of detection (LOD) and limit of quantification (LOQ) ere less than 36.32 and 122.09ng. The relative standard deviations (RSDs) for intra-day and inter-day repeatability were no more than 3.23% and 4.85%, respectively. The sample was stable for at least 48hr. The spike recoveries of nine components were 96-106%. The established method was successfully applied to determine nine components in thirteen samples from different locations. The results showed that the newly developed HPLC-DAD method was sensitive, precise, and accurate and could be used for quality control of P. cablin.