Journal
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
Volume 31, Issue 18, Pages 2752-2761Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/10826070802388326
Keywords
matrine; oxymatrine; RP-HPLC; Sophora Flavescens Ait.
Funding
- Center for Advanced Bioseparation Technology and Inha University, Korea
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In the present work, a reversed phase high performance liquid chromatographic method (HPLC) has been developed and validated for analysis of matrine and oxymatrine in Sophora Flavescens Ait. HPLC separation of the alkaloids was performed on a C18 column. A mobile phase composed of methanol-water-trifluoroacetic in a ratio of 16:84:0.002 (v/v) was found to be the most suitable mobile phase for this separation. The extracted amounts are 0.0091 and 0.15mg/g and the relative standard deviations were 3.3% for the matrine and 2.9% for the oxymatrine analysis.
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