Journal
JOURNAL OF LIPID RESEARCH
Volume 55, Issue 2, Pages 289-298Publisher
ELSEVIER
DOI: 10.1194/jlr.M044826
Keywords
lipidomics; mass spectrometry; dry eye syndrome; meibum; tear lipidome; cholesteryl sulfates; O-acyl-omega-hydroxy fatty acids
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Funding
- Chinese Academy of Sciences [KYQY-162, Y265091891]
- Singapore National Research Foundation [NMRC/CSA/013/2009, NMRC/1206/2009, 2007-4]
- National University of Singapore
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The tear film covers the anterior eye and the precise balance of its various constituting components is critical for maintaining ocular health. The composition of the tear film amphiphilic lipid sublayer, in particular, has largely remained a matter of contention due to the limiting concentrations of these lipid amphiphiles in tears that render their detection and accurate quantitation tedious. Using systematic and sensitive lipidomic approaches, we validated different tear collection techniques and report the most comprehensive human tear lipidome to date; comprising more than 600 lipid species from 17 major lipid classes. Our study confers novel insights to the compositional details of the existent tear film model, in particular the disputable amphiphilic lipid sublayer constituents, by demonstrating the presence of cholesteryl sulfate, O-acyl-omega-hydroxy fatty acids, and various sphingolipids and phospholipids in tears. The discovery and quantitation of the relative abundance of various tear lipid amphiphiles reported herein are expected to have a profound impact on the current understanding of the existent human tear film model.
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