Journal
JOURNAL OF LEUKOCYTE BIOLOGY
Volume 95, Issue 6, Pages 853-866Publisher
FEDERATION AMER SOC EXP BIOL
DOI: 10.1189/jlb.0113053
Keywords
lymphocyte development; pro-B cells; Sca-1; IFN-gamma; IL-7
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Funding
- Integrated Center for Research and Therapy (IFTZ) of Innsbruck Medical University
- doctorate program in Molecular Cell Biology and Oncology (MCBO) - Austrian Science Fund (FWF)
- Austrian Cancer Society/Tirol
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STAT1 serves as an important regulator in the response to pathogens, oncogenic transformation, and genotoxic insults. It exerts these effects by shaping the innate and adaptive immune response and by participating in genotoxic stress pathways, leading to apoptosis and inhibition of cell proliferation. We have investigated the role of STAT1 in hematopoietic toxicity induced by doxorubicin in STAT1-proficient and-deficient mice. Whereas the early genotoxic effect of doxorubicin did not depend on STAT1, expression of STAT1 was required for efficient B lymphocyte re-population in the recovery phase. We found a lower abundance of lymphocyte precursors in the BM of STAT1-deficient animals, which was particularly evident after doxorubicin-induced hematopoietic toxicity. In accordance, colony-forming assays with STAT1-deficient BM cells revealed a decreased number of pre-B colonies. Differentiation from the pro-B to the pre-B stage was not affected, as demonstrated by unaltered differentiation of purified B cell precursors from BM in the presence of IL-7. With the exception of Sca-1, expression of genes implicated in early lymphocyte development in pro-B cells did not depend on STAT1. Our findings indicate a specific requirement for STAT1 in lymphoid development before differentiation to pre-B cells, which becomes particularly apparent in the recovery phase from doxorubicin-induced hematopoietic toxicity.
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