Journal
JOURNAL OF LEUKOCYTE BIOLOGY
Volume 89, Issue 5, Pages 649-659Publisher
WILEY
DOI: 10.1189/jlb.0410195
Keywords
Drosophila; zebrafish; plasmatocyte; macrophage; immunity
Categories
Funding
- Sigrid Juselius Foundation
- Academy of Finland
- Foundation for Pediatric Research
- Tampere Tuberculosis Foundation
- Emil Aaltonen Foundation
- Swedish Research Council
- Swedish Cancer Society
- Pirkanmaa Hospital District
- Biocenter Finland
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Phagocytosis is an ancient cellular process that plays an important role in host defense. In Drosophila melanogaster phagocytic, macrophage-like hemocytes recognize and ingest microbes. We performed an RNAi-based in vitro screen in the Drosophila hemocyte cell line S2 and identified Abi, cpa, cofilin regulator 14-3-3 zeta, tlk, CG2765, and CG15609 as mediators of bacterial phagocytosis. Of these identified genes, 14-3-3 zeta had an evolutionarily conserved role in phagocytosis: bacterial phagocytosis was compromised when 14-3-3 zeta was targeted with RNAi in primary Drosophila hemocytes and when the orthologous genes Ywhab and Ywhaz were silenced in zebrafish and mouse RAW 264.7 cells, respectively. In Drosophila and zebrafish infection models, 14-3-3 zeta was required for resistance against Staphylococcus aureus. We conclude that 14-3-3 zeta is essential for phagocytosis and microbial resistance in insects and vertebrates. J. Leukoc. Biol. 89: 649-659; 2011.
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