Journal
JOURNAL OF LEUKOCYTE BIOLOGY
Volume 91, Issue 3, Pages 461-467Publisher
WILEY
DOI: 10.1189/jlb.0711361
Keywords
RNA interference; immune regulation; galectins
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Funding
- Norwegian Cancer Society
- Norwegian Radium Hospital
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DCs are specialized APCs capable of inducing T cell activation as well as promoting tolerance. Although Gal, a family of beta-galactoside-binding proteins, were found to affect immunity, little is known about the contribution of DC-expressed Gal on T cell activation. Here, we show that human imDCs and mDCs constitutively express Gal-1, Gal-3, Gal-8, and Gal-9 at mRNA and protein levels. Two of the most abundant Gal-Gal-1 and Gal-3-ere highly expressed and detected on the cell surface of DCs. In contrast to Gal-8, knockdown of Gal-1 or Gal-3 in DCs enhanced allogeneic T cell responses. This was observed with imDCs and mDCs, but the effects were more pronounced with imDCs. Furthermore, allogeneic CD4(+) T cells incubated with Gal-1 or Gal-3 knockdown DCs produced more IFN-gamma and less IL-10 than did control cells. The percentage of apoptotic T cells was significantly higher in cultures with control DCs than that with Gal-1 or Gal-3 knockdown DCs. Collectively, the data indicate that DC-expressed Gal-1 and Gal-3 are regulatory molecules that favor the inhibition of T cell activation. Furthermore, the data provide a new mechanism for the poor capacity of imDCs to stimulate T cells. J. Leukoc. Biol. 91: 461-467; 2012.
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