Journal
JOURNAL OF LEUKOCYTE BIOLOGY
Volume 88, Issue 3, Pages 517-522Publisher
WILEY
DOI: 10.1189/jlb.0310134
Keywords
keratitis; extracellular matrix; matrix metalloproteinase
Categories
Funding
- National Institute of Health [EY10320, EY11373, EY11845, EY12486, K08 EY014912]
- Research to Prevent Blindness Foundation
- Ohio Lions Eye Research Foundation
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Keratocan and lumican are small, leucine-rich repeat KSPGs in the extracellular matrix (ECM) of the mammalian cornea, whose primary role is to maintain corneal transparency. In the current study, we examined the role of these proteoglycans in the breakdown of the chemokine gradient and resolution of corneal inflammation. LPS was injected into the corneal stroma of C57BL/6 mice, and corneal extracts were examined by immunoblot analysis. We found reduced expression of the 52-kD keratocan protein after 6 h and conversely, increased expression of 34/37 kD immunoreactive products. Further, appearance of the 34/37-kD proteins was dependent on neutrophil infiltration to the cornea, as the appearance of these products was coincident with neutrophil infiltration, and the 34/37-kD products were not detected in explanted corneas or in CXCR2(-/-) corneas with deficient neutrophil recruitment. Furthermore, the 34/37-kD products and CXCL1/KC were detected in the anterior chamber, into which the corneal stroma drains; and CXCL1/KC was elevated significantly in keratocan(-/-) and lumican(-/-) mice. Together, these findings indicate that the inflammatory response in the cornea is regulated by proteoglycan/CXCL1 complexes, and their diffusion into the anterior chamber is consistent with release of a chemokine gradient and resolution of inflammation. J. Leukoc. Biol. 88: 517-522; 2010.
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