Journal
JOURNAL OF LEUKOCYTE BIOLOGY
Volume 84, Issue 1, Pages 124-133Publisher
FEDERATION AMER SOC EXP BIOL
DOI: 10.1189/jlb.1107744
Keywords
tolerance; cytokine deviation; hyporesponsiveness; immunotherapy
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Funding
- Arthritis Research UK [17750] Funding Source: Medline
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Promising immunotherapeutic tools for T cell-mediated pathologies are alternatively activated dendritic cells (aaDC), which exert their effect through the regulation and tolerization of T cells. As naive and memory T cells have different susceptibilities to tolerogenic signals, it is important to understand the modulatory effects of aaDC on these T cell subsets. We have examined regulation of naive and memory CD4(+) T cells by human aaDC generated with dexamethasone, the active form of vitamin D3, 1 alpha,25-dihydroxyvitamin D3, and LPS. Although aaDC induced low, primary, allogeneic responses by naive and memory T cells, aaDC regulated the differentiation of these T cell subsets in a distinct manner. Naive T cells primed by aaDC retained a strong, proliferative capacity upon restimulation but were skewed toward a low IFN-gamma/high IL-10 cytokine profile. In contrast, memory T cells primed by aaDC became hyporesponsive in terms of proliferation and cytokine production. Induction of anergy in memory T cells by aaDC was not a result of the presence of CD25(hi) regulatory T cells and could be partially reversed by IL-2. Both T cell subsets acquired regulatory activity and inhibited primary CD4 and CD8 responses. Addition of exogenous IL-12p70 during T cell priming by aaDC prevented anergy induction in memory T cells and cytokine polarization in naive T cells, indicating that the lack of IL-12p70 is a key feature of aaDC. Our finding that aaDC differentially regulate naive and memory T cells is important for understanding and maximizing the therapeutic potential of aaDC.
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