Journal
JOURNAL OF LEUKOCYTE BIOLOGY
Volume 84, Issue 4, Pages 1075-1081Publisher
FEDERATION AMER SOC EXP BIOL
DOI: 10.1189/jlb.0706462
Keywords
sialic acid cell trafficking; inflammation
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Funding
- NHLBI NIH HHS [R01 HL086933, HL086933] Funding Source: Medline
- NIAID NIH HHS [AI42818] Funding Source: Medline
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Endogenous polymorphonuclear leukocyte (PMN)-associated sialidase activity enhances PMN adhesion to and migration across the endothelium through the removal of sialylated cell-surface residues. We tested the hypothesis that PMNs also express sialyltransferase (ST) activity that restores sialyl residues to the PMN surface. We developed a highly sensitive fluorometric assay to demonstrate that intact human PMNs can mediate and accept sialyl residue transfer. This ST activity is inhibited by a ST inhibitor, CMP, which also inhibits the transendothelial migration of PMNs in response to IL-8 in vitro and in vivo. We conclude that intact PMNs express sialidase and ST activities that permit rapid modulation of their surface sialylation and their ability to adhere to and migrate across the endothelium.
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