Journal
JOURNAL OF INVESTIGATIVE DERMATOLOGY
Volume 133, Issue 9, Pages 2229-2236Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/jid.2013.70
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Funding
- Wellcome Trust
- Caring Matters Now charity
- Wellbeing of Women
- MRC
- Great Ormond Street Hospital Children's Charity
- MRC [G1001689] Funding Source: UKRI
- Great Ormond Street Hospital Childrens Charity [V1266] Funding Source: researchfish
- Medical Research Council [G1001689] Funding Source: researchfish
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Congenital melanocytic nevi (CMN) can be associated with neurological abnormalities and an increased risk of melanoma. Mutations in NRAS, BRAF, and Tp53 have been described in individual CMN samples; however, their role in the pathogenesis of multiple CMN within the same subject and development of associated features has not been clear. We hypothesized that a single postzygotic mutation in NRAS could be responsible for multiple CMN in the same individual, as well as for melanocytic and nonmelanocytic central nervous system (CNS) lesions. From 15 patients, 55 samples with multiple CMN were sequenced after site-directed mutagenesis and enzymatic digestion of the wild-type allele. Oncogenic missense mutations in codon 61 of NRAS were found in affected neurological and cutaneous tissues of 12 out of 15 patients, but were absent from unaffected tissues and blood, consistent with NRAS mutation mosaicism. In 10 patients, the mutation was consistently c.181C>A, p.Q61K, and in 2 patients c.182A>G, p.Q61R. All 11 non-melanocytic and melanocytic CNS samples from 5 patients were mutation positive, despite NRAS rarely being reported as mutated in CNS tumors. Loss of heterozygosity was associated with the onset of melanoma in two cases, implying a multistep progression to malignancy. These results suggest that single postzygotic NRAS mutations are responsible for multiple CMN and associated neurological lesions in the majority of cases.
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