Journal
JOURNAL OF INVESTIGATIVE DERMATOLOGY
Volume 133, Issue 3, Pages 768-775Publisher
ELSEVIER SCIENCE INC
DOI: 10.1038/jid.2012.357
Keywords
-
Categories
Funding
- German Cancer Aid (Melanoma Research Network)
- DKFZ-MOST-Program [Ca-135]
Ask authors/readers for more resources
MicroRNAs are small noncoding RNAs that regulate gene expression and have important roles in various types of cancer. Previously, miR-137 was reported to act as a tumor suppressor in different cancers, including malignant melanoma. In this study, we show that low miR-137 expression is correlated with poor survival in stage IV melanoma patients. We identified and validated two genes (c-Met and YB1) as direct targets of miR-137 and confirmed two previously known targets, namely enhancer of zeste homolog 2 (EZH2) and microphthalmia-associated transcription factor (MITF). Functional studies showed that miR-137 suppressed melanoma cell invasion through the downregulation of multiple target genes. The decreased invasion caused by miR-137 overexpression could be phenocopied by small interfering RNA knockdown of EZH2, c-Met, or Y box-binding protein 1 (YB1). Furthermore, miR-137 inhibited melanoma cell migration and proliferation. Finally, miR-137 induced apoptosis in melanoma cell lines and decreased BCL2 levels. In summary, our study confirms that miR-137 acts as a tumor suppressor in malignant melanoma and reveals that nniR-137 regulates multiple targets including c-Met, YB1, EZH2, and MITF. Journal of Investigative Dermatology (2013) 133, 768-775; doi:10.1038/jid.2012.357; published online 15 November 2012
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available