Journal
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
Volume 2015, Issue -, Pages -Publisher
HINDAWI LTD
DOI: 10.1155/2015/481405
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Funding
- Natural Science Foundation of Shandong Province [ZR2011HL013]
- Science and Technology Foundation of Shihezi [2014QY16]
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Objective. This study aimed to evaluate the protective effect of kaempferol against myocardial ischemia/reperfusion (I/R) injury in rats. Method. Left ventricular developed pressure (LVDP) and its maximum up/down rate (+/-dp/dt(max)) were recorded as myocardial function. Infarct size was detected with 2,3,5-triphenyltetrazolium chloride staining. Cardiomyocyte apoptosis was determined using terminal deoxynucleotidyl nick-end labeling (TUNEL). The levels of creatine kinase (CK), lactate dehydrogenase (LDH), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione/glutathione disulfide (GSH/GSSG) ratio, and tumor necrosis factor-alpha (TNF-alpha) were determined using enzyme linked immunosorbent assay (ELISA). Moreover, total glycogen synthase kinase-3 beta (GSK-3 beta), phospho-GSK-3 beta (P-GSK-3 beta), precaspase-3, cleaved caspase-3, and cytoplasm cytochrome C were assayed using Western blot analysis. Results. Pretreatment with kaempferol significantly improved the recovery of LVDP and +/-dp/dt(max), as well as increased the levels of SOD and P-GSK-3 beta and GSH/GSSG ratio. However, the pretreatment reduced myocardial infarct size and TUNEL-positive cell rate, as well as decreased the levels of cleaved caspase-3, cytoplasm cytochrome C, CK, LDH, MDA, and TNF-alpha. Conclusion. These results suggested that kaempferol provides cardioprotection via antioxidant activity and inhibition of GSK-3 beta activity in rats with I/R.
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