Journal
JOURNAL OF INVESTIGATIVE DERMATOLOGY
Volume 130, Issue 3, Pages 755-762Publisher
ELSEVIER SCIENCE INC
DOI: 10.1038/jid.2009.343
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Funding
- Austrian Science Fund [L120-B13]
- Kompetenzzentrum Medizin Tirol-CE-MIT [KMT-03b]
- COMET K1 Center Oncotyrol [1 3]
- Federal Ministry for Transport, Innovation & Technology (BMVIT)
- Federal Ministry of Economics & Labour/Federal ministry of Economy, Family & Youth (BMWA/BMWFJ)
- Tiroler Zukunitsstiftung (TZS)
- TILAK hospital holding company
- Innsbruck Medical University [MFI-9442]
- National institutes of Health [AI 057158, AI 13013, AI 40874]
- Center for AIDS Vaccine Discovery
- Canadian Histiocytosis Association
- Austrian Science Fund (FWF) [P 21487] Funding Source: researchfish
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Antigen-presenting cells can capture antigens that are deposited in the skin, including vaccines given subcutaneously. These include different dendritic cells (DCs) such as epidermal Langerhans cells (LCs), dermal DCs, and dermal langerin(+) DCs. To evaluate access of dermal antigens to skin DCs, we used mAb to two C-type lectin endocytic receptors, DEC-205/CD205 and langerin/CD207. When applied to murine and human skin explant cultures, these mAbs were efficiently taken up by epidermal LCs. In addition, anti-DEC-205 targeted langerin(+) CD103(+) and langerin(-) CD103(-) mouse dermal DCs. Unexpectedly, intradermal injection of either mAb, but not isotype control, resulted in strong and rapid labeling of LCs in situ, implying that large molecules can diffuse through the basement membrane into the epidermis. Epidermal LCs targeted in vivo by ovalbumin-coupled anti-DEC-205 potently presented antigen to CD4(+) and CD8+ T cells in vitro. However, to our Surprise, LCs targeted through langerin were unable to trigger T-cell proliferation. Thus, epidermal LCs have a major function in uptake of lectin-binding antibodies under standard vaccination conditions.
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